Metalloprotein Design & Engineering
نویسنده
چکیده
Metalloproteins play important roles in biology. They account for about 1/3 of structurally characterized proteins and about 1/2 of all proteins.1 The availability of different metal ions with various redox states, ligands, and geometries about the metal ion allows for fine-tuning of the reactivity of proteins at the highest level. As shown in other sections of this encyclopedia, studies of native metalloproteins and their variants have provided numerous insights. Design and engineering of metalloproteins can serve as a touchstone whereby the principles obtained from the studies are tested, and help determine whether the necessary features identified from the studies are sufficient to confer the structure and function of the proteins. Since it is a bottom-up approach of building the proteins that is complementary to the top-down approach of native protein studies, it can offer new insights. Furthermore, it is possible to design metalloproteins with unprecedented structural and functional properties. Finally, most designed proteins are smaller than the native proteins, making them easier for practical applications. A number of excellent reviews on metalloprotein design and engineering have appeared in the literature.2–28 This review will focus mainly on advances in creating new metalbinding sites in proteins.
منابع مشابه
Advances in metalloprotein and redox protein design
Metalloprotein and redox protein design are rapidly advancing toward the chemical synthesis of novel proteins that have predictable structures and functions. Current data demonstrate a breadth of successful approaches to metallopeptide and metalloprotein design based on de novo, rational and combinatorial strategies. These sophisticated synthetic analogs of natural proteins constructively test ...
متن کاملThe New York Section of the American Chemical Society.
Featured Keynote Speaker Prof. Brian Gibney CUNY Graduate Center While DNA is the blueprint for the cell, it is the proteins that do the bulk of the work. About 30% of proteins require metal ions for proper function and their dysfunction leads to human disease. The Gibney Lab takes a constructive approach toward understanding metalloprotein structurefunction relationships. Using inorganic chemi...
متن کاملGeneration of novel functional metalloproteins via hybrids of cytochrome c and peroxidase.
The continued interest in protein engineering has led to intense efforts in developing novel stable enzymes, which could not only give boost to industrial and biomedical applications, but also enhance our understanding of the structure-function relationships of proteins. We present here the generation of three hybrid proteins of cytochrome c (cyt c) and peroxidase via structure-based rational m...
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Zinc is one of the most important metal ions found in proteins performing specific functions associated with life processes. Coordination geometry of the zinc ion in the active site of the metalloprotein-ligand complexes poses a challenge in determining ligand binding affinities accurately in structure-based drug design. We report here an all atom force field based computational protocol for es...
متن کاملMetalloprotein design using genetic code expansion.
More than one third of all proteins are metalloproteins. They catalyze important reactions such as photosynthesis, nitrogen fixation and CO2 reduction. Metalloproteins such as the olfactory receptors also serve as highly elaborate sensors. Here we review recent developments in functional metalloprotein design using the genetic code expansion approach. We show that, through the site-specific inc...
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